The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products and solutions were digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A degree mutation was released by the https://louisb221mwg3.birderswiki.com/user