A few plasmids (pGL1124, pGL1224 and pGL1217) ended up produced to allow the substitution of one allele of CYC9 Lister 427 cells. Trypanosomes were being transfected with Every single plasmid separately, after which subjected to your next transfection with one of several other plasmids, with a distinct resistance gene, with https://griffinkfwma.tinyblogging.com/guaiapate-an-overview-74424072