I agree with Biffo that you'd be unlikely to confuse "No." with the term "No" at the time you think about it. But I do sometimes encounter a break up-2nd hesitation when looking at a sentence with "No.", particularly if there is a space amongst it and the subsequent selection: https://90sgirlgroupnoscrubs97428.izrablog.com/36937629/the-smart-trick-of-no-scrub-cover-that-nobody-is-discussing